Molecular properties of short chain acyl thioesters of acyl carrier protein.
نویسندگان
چکیده
منابع مشابه
Versatility of acyl-acyl carrier protein synthetases.
The acyl carrier protein (ACP) requires posttranslational modification with a 4'-phosphopantetheine arm for activity, and this thiol-terminated modification carries cargo between enzymes in ACP-dependent metabolic pathways. We show that acyl-ACP synthetases (AasSs) from different organisms are able to load even, odd, and unnatural fatty acids onto E. coli ACP in vitro. Vibrio harveyi AasS not o...
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/3-Hydroxyacyl acyl carrier protein (ACP) dehydrase has been purified 2900-fold from extracts of Escherichia coli. The enzyme catalyzes the reversible dehydration of /3-hydroxyacyl-ACP thioesters to yield specifically tram-2-enoylACP products. It is active with frans-2-enoyl-ACP thioesters of chain lengths from 4 through 16 carbon atoms. The enzyme catalyzes the hydration of cis-5frans-2-dodeca...
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Closfridium bufyricum grown in medium supplemented with radioactive pantothenic acid incorporates this compound into both protein and nonprotein fractions of the cell. Acyl carrier protein (ACP) is the major protein into which pantothenic acid is incorporated. C. bufyricum ACP was isolated as a homogeneous protein, as judged by column chromatography on DEAE-Sephadex, disc gel electrophoresis, a...
متن کاملEnzymatic desaturation of stearyl acyl carrier protein.
Stearyl acyl carrier protein, chemically prepared from 1-W stearic acid and Escherichia co2i acyl carrier protein, is desaturated to oleic acid by extracts of photoauxotrophic Euglena gracilis and of spinach. The soluble stearyl acyl carrier protein desaturase system from Eug~ena has been separated into three components, a reduced triphosphopyridine nucleotide oxidase, the desaturase, and a non...
متن کاملA Cerulenin Insensitive Short Chain 3-Ketoacyl-Acyl Carrier Protein Synthase in Spinacia oleracea Leaves.
A cerulenin insensitive 3-ketoacyl-acyl carrier protein synthase has been assayed in extracts of spinach (Spinacia oleracea) leaf. The enzyme was active in the 40 to 80% ammonium sulfate precipitate of whole leaf homogenates and catalyzed the synthesis of acetoacetyl-acyl carrier protein. This condensation reaction was five-fold faster than acetyl-CoA:acyl carrier protein transacylase, and the ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1982
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)34627-1